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    Current location:index > Homocysteine Assay Kit

    Biochemical diagnostic reagents

    Product name:

    Homocysteine Assay Kit

    Since 1990s, It is widely believed that an elevated level of homocysteine is an independent risk factor for cardiovascular disease.  Recent studies have also demonstrated a strong correlation between elevated Hcy levels and diseases such as diabetes, Alzheimers osteoporosis, and renal failure.

    The Clinisciences enzymatic cycling Homocysteine assay is a liquid stable 2-part system which is intended for in vitro quantitative determination of total L-homocysteine in human serum and plasma. The assay provides a fast, accurate cost effective alternative to cumbersome and expensive immunochemical methods and is not interfered by the endogenous cystathionine and therefore can be used with confidence in patients with renal disease.

    In the assay, oxidized Hcy is first reduced to free Hcy which then reacts with a co-substrate, SAM, catalyzed by a homocysteine S-methyltransferase. The co-substrate conversion product is amplified by coupled enzymatic cycling reactions. The tHcy level in the sample is indirectly proportional to the amount of NADH conversion to NAD+ (ΔA340nm).

     



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